Error Message: “The application vistualization sequencer did not shutdown successgfully the last time the sequencer was user. Please reboot your sustem before using the Application Virtualization sequencer”

Resolution: Simply delete the following registry key
[HKEY_LOCAL_MACHINESOFTWAREWow6432NodeMicrosoftSoftGrid4.5SequencerSeqLastStartTime]=dword:4fd5f977